1.) Course Description
This two-day hands-on workshop offers demonstration of protein purification using monolithic columns. You will obtain detailed information about chromatographic purification strategies for proteins, how to perform capture and polishing steps on monolithic columns tailored for plasma protein separation from different sample types and practical instruction on applying analytical LC methods for in-process monitoring during chromatography runs. Additionally, you will learn how to perform continuous chromatographic operations using monolithic chromatographic media and how to prepare functional immobilized enzyme reactors (IMERs) using monolithic supports, including hands-on testing of their enzymatic activity. The training includes live demonstrations in a BSL laboratory, where real samples will be processed under the guidance of experts in monolith technology and protein purification.
2.) Who Should Attend?
◾︎Process Development
◾︎Research & Development
◾︎Downstream process
3.) Key Learnings
◾︎How to implement novel column formats and ligand options designed to ensure highly specific binding of proteins during purification.
◾︎How to successfully prepare the sample and choose chromatographic conditions to selectively bind target-specific plasma proteins while preserving physiological activity and avoiding unwanted activation.
◾︎How to optimize the right chromatographic conditions for the Immunoglobulin G polishing step with monolithic columns that maintain Immunoglobulin G subclass composition, effectively remove contaminants, and preserve therapeutic properties.
◾︎How to perform continuous chromatographic operations using monolithic chromatographic media and a straightforward transfer method from a 1 ml system to a continuous annular chromatography system.
◾︎How to determine protein concentration and monitor process variability by designing CIMac-based analytical methods for in-process chromatography of proteins.
◾︎How to prepare and characterize Immobilized Enzyme Reactors (IMERs) on monolithic supports that deliver performance comparable to solutions and eliminate the need for enzyme removal.